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Summary of Structures, Activities and Technologies 2012

2012 Summary (PDF)

New techniques and technology for membrane protein research

I. Production of Quality Membrane Proteins for Crystallization (PDF)

II. Yeast membrane proteomics project (PDF)

III. Selecting optimum integral membrane protein targets (PDF)

IV. Cell Free expression integral membrane protein (PDF)

V. GFP Fusion assay to identify well-expressed membrane proteins (PDF)

VI. Gene redesign and Synthesis

VII. A High throughput assay for membrane protein stabilization by ligands, buffers detergents, lipids. (PDF#1) (PDF#2) (PDF#3) (PDF#4)

VIII. High quality of eukaryotic membrane proteins in S. cerevisciae. (PDF#1) (PDF#2)

IX.  Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells. 2011 Sep 8. [Epub ahead of print]

X.  Overexpressing Human Membrane Proteins in Stably Transfected and Clonal Human Embryonic Kidney 293S Cells.

Publications


1. Vardy, E., Steiner-Mordoch, S., Schuldiner, S. (2005). Characterization of bacterial drug antiporters homologous to mammalian neurotransmitter transporters. J Bacteriol, 187, 7518-7525. (PMID: 16237035 / PMCID: PMC1272986)

2. Guan, L., Mirza, O., Verner, G., Iwata, S., Kaback, H.R. (2007). Structural determination of wild-type lactose permease. Proc Natl Acad Sci U S A. 104, 15294-8. (PMID: 17881559 / PMCID: PMC2000551).

3. Menuz, K., Stroud, R.M., Nicoll, R.A., Hays, F.A. (2007). TARP auxiliary subunits switch AMPA receptor antagonists into partial agonists. Science. 318, 815-7. (PMID:17975069)

4. Minor, D.L., Jr., (2007). The neurobiologist's guide to structural biology: A Primer on why macromolecular structure matters and how to evaluate macromolecular structural data. Neuron 54, 511-533. (PMID: 17521566 / PMCID: PMC3011226)

5. Nie, Y., Ermolova, N., Kaback, H.R. (2007). Site-directed alkylation of LacY: effect of the proton electrochemical gradient. J Mol Biol. 374, 356-64. (PMID: 17920075 / PMCID: PMC2224895).

6. Savage, D. F., Anderson, C.L., Robles-Colmenares, Y., Newby, Z., Stroud, R.M. (2007). Cell-free complements in vivo expression of the E. coli membrane proteome. Protein Science. 16, 966-76. (PMID: 17456747 / PMCID: PMC2206641)

7. Schuldiner, S. (2007). When biochemistry meets structural biology: the cautionary tale of EmrE. TIBS. 32, 252-8. (PMID: 17452106)

8. Smirnova, I., Kasho, V., Choe, J.Y., Altenbach, C., Hubbell, W.L., Kaback, H.R. (2007). Sugar binding induces an outward facing conformation of LacY. Proc Natl Acad Sci U S A. 104, 16504-9. (PMID: 17925435 / PMCID: PMC2034228).

9. Smirnova, I., Kasho, V., Kaback, H.R. (2007). Protonation and sugar binding to LacY. Proc Natl Acad Sci USA. 105, 8896-8901. (PMID: 18567672 / PMCID: PMC3021437)

10. Adam, Y., Edwards, R.H., Schuldiner, S. (2008). Expression and function of the rat vesicular monoamine transporter 2. Am J Physiol Cell Physiol. 294, C1004-11. (PMID: 18287335).

11. Kelly, L., Pieper, U., Eswar, N., Hays, F., Li, M., Roe-Zurz. Z., Kroetz, D., Giacomini, K., Stroud, R.M., Sali, A. (2008). A taxonomic profile of the membrane protein universe. Submitted to: Genome Biology

12. Lee, J.K., Belogrudov, G.I., Stroud, R.M. (2008). Crystal structure of bovine mitochondrial factor B at 0.96-Å resolution. PNAS 105, 13381-13384. (PMID: 18768789)

13. Li, M., Hays, F.A., Roe-Zurz, Z., Vuong, L., Kelly, L., Robbins, R., Ho, C., Pieper, U., O'Connell III, J., Miercke, L.J.W., Giacomini, K.M., Sali, A., Stroud, R.M. (2008). Eukaryotic Integral Membrane Protein Production For Structural Genomics. J. Mol Biol. 385, 820-30.

14. Newby, Z., O'Connell III, J., Robles-Colmenares, Y., Khademi, S., Miercke, L.J.W., Stroud, R.M. (2008). Crystal structure of the aquaglyceroporin PfAQP form the malarial parasite P.falciparum. Nature Structural Biology. 15, 619-25. (PMID: 18500352 / PMCID: PMC2568999).

15. Nie, Y., Sabetfard, F.E., Kaback, H.R. (2008). The Cys154→Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. J Mol Biol. 379, 695-703. (PMID: 18485365 / PMCID: PMC2769995)

16. Smirnova, I., Kasho, V., Kaback, H. R. (2008). Protonation and sugar binding to LacY. PNAS. 105, 8896-901. (PMID: 18567672 / PMCID: PMC3021437)

17. Zhou, Y., Guan, L., Freites, J.A., Kaback, H.R. (2008). Opening and closing of the periplasmic gate in lactose permease. Proc Natl Acad Sci U S A. 105, 3774-8. (PMID: 18319336 / PMCID: PMC2268789).

18. Bassik, M.C., Lebbink, R.J., Churchman, L.S., Ingolia, N.T., Patena, W., LeProust, E.M., Schuldiner, M., Weissman, J.S., McManus, M.T. (2009). Rapid creation and quantitative monitoring of high coverage shRNA libraries. Nature Methods. 6:443-5. (PMCID: PMC2783737) *corresponding author

19. Garcia-Celma, J.-J., Smirnova, I.N., Kaback, H.R., Fendler, K. (2009). Electrophysiological characterization of LacY. Proc Natl Acad Sci USA. 106, 7373-8. (PMID: 19383792 / PMC2671326)

20. Guan, L., Kaback, H.R. (2009). Properties of a LacY efflux mutant. Biochemistry. 48, 9250-9255. (PMC2754601 / NIHMS142923)

21. Hammon, J., Palanivelu, D.V., Chen, J., Patel, C., Minor, D.L., Jr., (2009). A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms. Protein Sci. 18, 121-33. (PMID: 19177357 / PMCID: PMC2708023)

22. Hays, F.A., Roe-Zurz, Z., Li, M., Kelly, L., Gruswitz, F., Sali, A., Stroud, R.M. (2009). Ratiocinative Screen of Eukaryotic Integral Membrane Protein Expression and Solubilization for Structure Determination. J Struct Func Genomics. 10, 9-16. (PMID: 19031011 / PMCID: PMC2756966)

23. Ho, J.D., Yeh, R., Sandstrom, A., Chorny, I., Harries, W.E.C., Robbins, R.A., Miercke, L. J.W., Stroud, R.M. (2009). Crystal Structure of Human Aquaporin 4 at 1.8Å and its mechanism of conductance. PNAS. 106, 7437-42. (PMID: 19383790 / PMCID: PMC2678640)

24. Li, M., Hays, F. A., Roe-Zurz, Z., Vuong, L., Kelly, L., Ho, C. M., Robbins, R. M., Pieper, U., O'Connell, J. D., 3rd, Miercke, L. J., Giacomini, K.M., Sali, A., Stroud, R.M. (2009). Selecting optimum eukaryotic integral membrane proteins for structure determination by rapid expression and solubilization screening. J Mol Biol. 385, 820-30. (PMCID: PMC2659619 / NIHMS93258)

25. Newby, Z.E.R., O'Connell III, J.D., Gruswitz, F., Hays, F.A., Harries, W.C., Harwood, I.M., Ho, J.D., Lee, J.K., Miercke, L.J.W., Savage, D.F., Stroud, R.M. (2009). A general protocol for the crystallization of membrane proteins for X-Ray structural investigation. Nature Protocols. 4, 619-37. (PMID: 19360018)

26. Smirnova, I., Kasho, V., Sugihara, J., Choe, J.-Y., Kaback, H.R. (2009). Residues in the H+ translocation site define the pKa for sugar binding to LacY. Biochemistry. 48, 8852-8860. (PMID: 19689129 / NIHMS 141552)

27. Smirnova, I., Kasho, V., Sugihara, J., Kaback, H.R. (2009). Probing of the rates of alternating access in LacY with Trp fluorescence. Proc Natl Acad Sci USA 106, 21561-6. (PMID: 19959662 / PMCID: PMC2799877)

28. Zhou, Y., Nie, Y., Kaback, H.R. (2009). Residues Gating the Periplasmic Pathway of LacY. J Mol Biol. 394, 219-25. (PMID: 19781551 / NIHMS148671)

29. Breslow, D.K., Collins, S.R., Bodenmiller, B., Aebersold, R., Simons, K., Shevchenko, A., Ejsing, C.S., Weissman, J.S. (2010). Orm family proteins mediate sphingolipid homeostasis. Nature. 463, 1048-53. (PMCID: PMC2877384 / NIHMS 166293)

30. Welch, M., Govindarajan S., Ness, J.E., Villalobos A., Gurney A., Jeremy Minshull, J., Gustafsson C. (2009). Design Parameters to Control Synthetic Gene Expression in Escherichia coli. PLoS ONE. 4, e7002 (PMID: 19759823)

31. Welch, M., Villalobos, A., Gustafsson, C., Minshull, J. (2009). You're one in a googol: optimizing genes for protein expression. J Royal Society. 4, S467-76. (PMID:19324676 PMCID: PMC2843954)

32. Chae, P. S., Gotfryd, K., Pacyna, J., Miercke, L. J., Rasmussen, S. G., Robbins, R. A., Rana, R. R., Loland, C.J., Kobilka, B., Stroud, R.M., Byrne, B., Gether, U., Gellman, S. H. (2010). Tandem facial amphiphiles for membrane protein stabilization. J Am Chem Soc. 132, 16750-2. (PMCID: PMC3050673 / NIHMS250722)

33. Classen, S., Rodic, I., Holton, J., Hura, G. L., Hammel, M. & Tainer, J. A. (2010). Software for the high-throughput collection of SAXS data using an enhanced Blu-Ice/DCS control system. J. Sync. Rad. 17, 774-781.

34. Duriseti, K.S., Goetz, D.H., Hostetter, D.R., Wei, Y., Craik, C.S. (2010). Antagonstic antiupar antibodies significantly inhibit uPAR-mediated cellular signaling and migration. J Biol Chem. 285, 26878-88. (PMID: 20501655 / PMCID: PMC2930687)

35. Egea, P.F., Stroud, R.M. (2010). Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes. Proc Natl Acad Sci USA. 107, 17182-7. (PMCID: PMC2951439)

36. Elbaz, Y., Danieli, T., Kanner, B.I.,Schuldiner, S. (2010). Expression of Neurotransmitter Transporters for Structural and Biochemical Studies. Protein Expres Purif. 73, 152-60. (PMCID: PMC2913170)

37. Garcia-Celma, J.J., Ploch, J., Smirnova, I., Kaback, H.R., Fendler, K. (2010). Delineating electrogenic reactions during lactose/H+ symport. Biochemistry. 49, 6115-21. (PMCID: PMC2907097)

38. Gros, Y., Schuldiner S. (2010). Directed evolution reveals hidden properties of VMAT, a neurotransmitter transporter. J Biol Chem. 285, 5076-84. (PMCID: PMC2836110)

39. Gruswitz, F., Chaudhary, S., Ho, J.D., Schlessinger, A., Pezeshki, B., Ho, C-M, Sali, A., Westhoff, C.M., Stroud, R.M. (2010). Function of human Rh based on structure of RhCG at 2.1Å. Proc Natl Acad Sci USA. 107, 9638-43. (PMCID: PMC2906887)

40. Hays, F., Roe-Zurz, Z., Stroud R. M. (2010). Overexpression and Purification of Integral Membrane Proteins in Yeast. Methods Enzymology. 470, 695-707. (PMID: 20946832)

41. Hoefer, N., Aragao, D., Caffrey, M. (2010). Crystallizing Transmembrane Peptides in Lipidic Mesophases. Biophys. J. 99, L23-5. (PMCID: PMC2913208)

42. Holton, J. M., Frankel, K. A. (2010). The minimum crystal size needed for a complete diffraction data set. Acta Cryst. D Biol Crystallogr. 66, 393-408. (PMCID: PMC2852304)

43. Kirian, R. A., Wang, X., Weierstall, U., Schmidt, K. E., Spence, J. C. H., Hunter, M., Fromme, P., White, T., Chapman, H. N., Holton, J. (2010). Femtosecond protein nanocrystallography: data analysis methods. Opt. Express. 18, 5713-5723. (PMID: 20389587 / NIHMSID 213857)

44. Lang, P. T., Ng, H.L., Fraser, J. S., Corn, J. E., Echols, N., Sales, M., Holton, J. M. & Alber, T. (2010). Automated electron-density sampling reveals widespread conformational polymorphism in proteins. Protein Sci. 19, 1420-1431. (PMC2974833)

45. Lee, J.K., Stroud, R.M. (2010). Unlocking the eukaryotic membrane protein structural proteome. Curr Opin Struct Biol. 4, 464-70. (PMID: 20739007/PMC in process)

46. Liu, Z., Madej, M.G., Kaback, H.R. (2010). Helix dynamics in LacY: helices II and IV. J Mol Biol. 396, 617-26. (PMID: 20043916 / PMCID: PMC3043081)

47. Luan, B., Carr, R., Caffrey, M., Aksimentiev, A. (2010). The effect of calcium on the conformation of cobalamin transporter BtuB. Proteins. 78, 1153-1162. (PMID: 19927326 / PMCID: PMC2903617)

48. Nie, Y., Kaback, H.R. (2010). Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane. Proc Natl Acad Sci. 107, 9903-8. (PMCID: PMC2906848)

49. Schlessinger, A., Matsson, P., Shima, J.E., Pieper, U., Yee, S.w., Kelly L, Stroud, R.M., Ferrin TTE, Giacomini KM, Sali A. (2010). Comparison of human solute carriers. Protein Sci. 19, 412-28. (PMID: 20052679 / PMCID: PMC2866268)

50. Chapman, H. N., Fromme, P., Barty, A., White, T. A., Kirian, R. A., Aquila, A., Hunter, M. S., Schulz, J., DePonte, D. P., Weierstall, U., Doak, R. B., Maia, F. R. N. C., Martin, A. V., Schlichting, I., Lomb, L., Coppola, N., Shoeman, R. L., Epp, S. W., Hartmann, R., Rolles, D., Rudenko, A., Foucar, L., Kimmel, N., Weidenspointner, G., Holl, P., Liang, M., Barthelmess, M., Caleman, C., Boutet, S., Bogan, M. J., Krzywinski, J., Bostedt, C., Bajt, S., Gumprecht, L., Rudek, B., Erk, B., Schmidt, C., Homke, A., Reich, C., Pietschner, D., Struder, L., Hauser, G., Gorke, H., Ullrich, J., Herrmann, S., Schaller, G., Schopper, F., Soltau, H., Kuhnel, K.-U., Messerschmidt, M., Bozek, J. D., Hau-Riege, S. P., Frank, M., Hampton, C. Y., Sierra, R. G., Starodub, D., Williams, G. J., Hajdu, J., Timneanu, N., Seibert, M. M., Andreasson, J., Rocker, A., Jonsson, O., Svenda, M., Stern, S., Nass, K., Andritschke, R., Schroter, C.-D., Krasniqi, F., Bott, M., Schmidt, K. E., Wang, X., Grotjohann, I., Holton, J. M., Barends, T. R. M., Neutze, R., Marchesini, S., Fromme, R., Schorb, S., Rupp, D., Adolph, M., Gorkhover, T., Andersson, I., Hirsemann, H., Potdevin, G., Graafsma, H., Nilsson, B. & Spence, J. C. H. (2011). Femtosecond X-ray protein nanocrystallography. Nature 470, 73-77. (PMID: 21293373)

51. Caffrey, M. (2011). Crystallizing Membrane Proteins for Structure-Function Studies Using Lipidic Mesophases. Biochem. Soc. Trans. 39, 725-32. (PMID: 21599641 / PMC in process)

52. Chaptal, V., Kwon, S., Sawaya, M.R., Guan, L., Kaback, H.R., Abramson, J. (2011). Crystal Structure of lactose permease in complex with an affinity inactivator yields new insight into sugar recognition. Proc Natl Acad Sci. USA. 108, 9361-6. (PMID: 21593407 / PMCID: PMC3111295)

53. Chaudary, S., Pak, J., Pedersen, B., Bang, L., Zhang, L., Ngaw, S., Sharma, V., and Stroud, R.M. (2011). Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells. Methods. (PMID: 21925269)

54. Chou, S., Krasileva, K. V., Holton, J. M., Steinbrenner, A. D., Alber, T. & Staskawicz, B. J. (2011). Hyaloperonospora arabidopsidis ATR1 effector is a repeat protein with distributed recognition surfaces. PNAS USA 108, 13323-13328. (PMCID: PMC3156156)

55. Duriseti, S., Goetz, D.H., Hostetter, D.R., LeBeau, A.M., Wei, Y., Craik, C.S. (2010). Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration. J Biol Chem. 285, 26878-88. (PMCID: PMC2930687)

56. Fraser, J. S., Van den Bedem, H., Samelson, A. J., Lang, P. T., Holton, J. M., Echols, N. & Alber, T. (2011). Accessing protein conformational ensembles using room-temperature X-ray crystallography. PNAS USA 108, 16247-16252. (PMCID: PMC3182744)

57. Hoefer, N., Aragao, D., Caffrey, M. (2011). The Lipidic Cubic Phase as a Membrane Mimetic. Biophys. J. 100: 2075.

58. Hofer, N., Aragao, D., J.A., Lyons, Caffrey, M. (2011). Membrane Protein Crystallization in Lipidic Mesophases. Hosting lipid affects on the crystallization and structure of a transmembrane peptide. Cryst. Growth Des. 11, 1182-1192. (Available at URL http://pubs.acs.org/doi/abs/10.1021/cg101384p)

59. Jiang, X., Nie, Y., Kaback, H.R. (2011). Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport. Biochemistry, 50, 1634-40. (PMID: 21254783 / PMCID: PMC3057939 [Available on 2012/3/15])

60. Kaback, H.R., Smirnova, I., Kasho, V., Nie, Y., Zhou, Y. (2011). The alternating access transport mechanism in LacY. J Membr Biol. 239, 85-93. (PMCID: PMC3030946)

61. Kim, J., Stroud, R.M. and Craik, C.S. (2011). Rapid identification of recombinant Fabs that bind to membrane proteins. Methods. (PMID: 21958987)

62. Kirian, R. A., White, T. A., Holton, J. M., Chapman, H. N., Fromme, P., Barty, A., Lomb, L., Aquila, A., Maia, F. R. N. C., Martin, A. V., Fromme, R., Wang, X., Hunter, M. S., Schmidt, K. E. & Spence, J. C. H. (2011). Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals. Acta Cryst. A 67, 131-140. (PMCID: PMC3066792)

63. Li, D., Lee, J., Caffrey, M. (2011). Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen. Cryst. Growth Des. 11, 530-537. (PMCID: PMC3131202)

64. Li, D., Caffrey, M. (2011). The Lipidic Cubic Phase as a Membrane Mimetic for Integral Membrane Protein Enzymes. PNAS. 108, 8639-44. (PMCID: PMC3102357)

65. Pye, V. E., Aragao, D., Lyons, J. A., Caffrey, M. (2011). Overview of International Conference on the Crystallization of Biological Macromolecules. Cryst. Growth Des. In press. DOI: 10.1021/cg101379p http://pubs.acs.org/doi/abs/10.1021/cg101379p

66. Rasmussen, S.G.F., DeVree, B.T., Zou, Y., Kruse, A.C., Chung, K.Y., Kobilka, T.S., Thian, F.S., Chae, P.S., Pardon, E., Calinski, D., Mathiesen, J.M., Shah, S.T.A., Lyons, J.A., Caffrey, M., Gellman, S.H., Steyaert, J., Skiniotis, G., Weis, W.I., Sunahara, R.K., Kobilka, B.K. (2011). Crystal structure of the β2 adrenergic receptor–Gs protein complex. Nature. 477, 549-555. (PMCID: PMC3184188)

67. Rosenbaum, D.M., Zhang, C., Lyons, J.A., Holl, R., Aragao, D., Arlow, D.H., Rasmussen, S.G., Choi, H.J., DeVree, B.T., et al. (2011). Structure and function of an irreversible agonist-b2 adrenoceptor complex. Nature. 469, 236-240. (PMCID: PMC3074335)

68. Shaya, D., Kreir, M., Robbins, R.A., Wong, S., Hammon, J., Bruggemann, A., and Minor, D.L. Jr. (2011). Voltage-gated sodium channel (NaV) protein dissection creates a set of functional 'pore-only' proteins. Proc Natl Acad Sci. USA. 108, 12313-8. (PMCID: PMC3145705)

69. Smirnova, I., Kasho, V., Kaback, H.R. (2011). Lactose permease and the alternating access mechanism. Biochemistry. 50(45): 9684-93. PMID: 21995338/PMCID: PMC3210931. http://www.ncbi.nlm.nih.gov/pubmed/21995338

70. Smirnova, I., Kasho, V., Sugihara, J., Kaback, H.R. (2011). Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding. PNAS. 108, 15147-15151. (PMID: 21896727 / PMCID: PMC3174601)

71. Spence, J. C. H., Kirian, R. A., Wang, X., Weierstall, U., Schmidt, K. E., White, T., Barty, A., Chapman, H. N., Marchesini, S. & Holton, J. (2011). Phasing of coherent femtosecond X-ray diffraction from size-varying nanocrystals. Opt. Express 19, 2866-2873. (PMID: 21369108)

72. Stepanov, S., Hilgart, M., Yoder, D. W., Makarov, O., Becker, M., Sanishvili, R., Ogata, C. M., Venugopalan, N., Aragão, D., Caffrey, M., Smith, J. L., Fischetti, R. F. (2011). Fast fluorescence techniques for crystallography beamlines. J. Appl. Cryst. 44, 772-778. [doi:10.1107/S0021889811016748]
http://journals.iucr.org/j/issues/2011/04/00/dz5227/dz5227.pdf

73. Stroud, R.M, Schertler, G.F. (2011). Membranes. Curr Opin Struct Biol. 21, 495-6. (PMID: 21875531)

74. Stroud, R.M. (2011). New tools in membrane protein determination. F1000 Biol Rep. 3:8. (PMID: 21655333 / PMCID: 3100781)

75. Chaudhary, S., Pak, J.E., Gruswitz, F., Sharma, V., Stroud, R.M. (2012). Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells. Nat Protoc. 7(3):453-66. PMID: 22322218.

76. Welch, M., Villalobos, A., Gustafsson, C., Minshull , J. (2011). Designing Genes for Successful Protein Expression. Methods Enzymology. 498, 43-66. (PMID: 21601673)

77. Wu, S., Avila-Sakar, A., Kim, J., Booth, D.S., Greenberg, C.H., Rossi, A., Liao, M., Li, X., Alian, A., Griner, S.L., Juge, N., Yu, Y., Mergel, C.M., Chaparro-Riggers, J., Strop, P., Tampé, R., Edwards, R.H., Stroud, R.M., Craik, C.S., Cheng, Y. Fabs enable single particle cryoEM studies of small proteins. Structure. 20(4): 582-92, 2012. (PMCID: PMC3322386)

78. Yang, D., Cwynar, V.A., Hart, D.J., Madanmohan, J., Lee, J., Lyons, J., Caffrey, M. (2012). Preparation of 1-Monoacylglycerols via the Suzuki-Miyaura Reaction: 2,3-Dihydroxypropyl (Z )-tetradec-7-enoate. Org. Synth. 89: 2012 In press.

79. Zhou, Y., Nie, Y., Kaback, H.R. (2009). Residues Gating the Periplasmic Pathway of LacY. J Mol Biol. 394, 219-25. (PMID: 19781551 / PMCID: PMC2784193)

80. Smirnova, I., Kasho, V., Sugihara, J., Kaback, H.R. (2009). Probing of the rates of alternating access in LacY with Trp fluorescence. Proc Natl Acad Sci USA 106, 21561-6. (PMID: 19959662 / PMCID: PMC2799877)

81. Smirnova, I., Kasho, V., Sugihara, J., Choe, J.-Y., Kaback, H.R. (2009). Residues in the H+ translocation site define the pKa for sugar binding to LacY. Biochemistry. 48, 8852-8860. (PMID: 19689129 / PMCID: PMC2769999)

82. Newby, Z.E.R., O'Connell III, J.D., Gruswitz, F., Hays, F.A., Harries, W.C., Harwood, I.M., Ho, J.D., Lee, J.K., Miercke, L.J.W., Savage, D.F., Stroud, R.M. (2009). A general protocol for the crystallization of membrane proteins for X-Ray structural investigation. Nature Protocols. 4, 619-37. (PMID: 19360018 / NIHMS: 151516)

83. Lyons, J.A., Aragao, D., Slattery, O., Pisliakoc, A.V., Soulimane, T., Caffrey, M. Structural insights into electron transfer in caa3-type cytochrome oxidase. Nature (2012) (doi:10.1038/nature11182)

84. Lomb, L., Barends, T. R. M., Kassemeyer, S., Aquila, A., Epp, S. W., Erk, B., Foucar, L., Hartmann, R., Rudek, B., Rolles, D., Rudenko, A., Shoeman, R. L., Andreasson, J., Bajt, S., Barthelmess, M., Barty, A., Bogan, M. J., Bostedt, C., Bozek, J. D., Caleman, C., Coffee, R., Coppola, N., DePonte, D. P., Doak, R. B., Ekeberg, T., Fleckenstein, H., Fromme, P., Gebhardt, M., Graafsma, H., Gumprecht, L., Hampton, C. Y., Hartmann, A., Hauser, G., Hirsemann, H., Holl, P., Holton, J. M., Hunter, M. S., Kabsch, W., Kimmel, N., Kirian, R. A., Liang, M., Maia, F. R. N. C., Meinhart, A., Marchesini, S., Martin, A. V., Nass, K., Reich, C., Schulz, J., Seibert, M. M., Sierra, R., Soltau, H., Spence, J. C. H., Steinbrener, J., Stellato, F., Stern, S., Timneanu, N., Wang, X., Weidenspointner, G., Weierstall, U., White, T. A., Wunderer, C., Chapman, H. N., Ullrich, J., Strüder, L. & Schlichting, I. Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser. Physical Review B 84, 214111, 2011. http://prb.aps.org/abstract/PRB/v84/i21/e214111

85. Schneider, E.L., Lee, M.S., Baharuddin, A., Goetz, D.H., Farady, C.J., Ward, M., Wang, C.I., Craik, C.S. A reverse binding motif that contributes to specific protease inhibition by antibodies. J Mol Biol 415(4):699-715, 2012. PMID: 22154938 / PMCID: PMC3268006

86. Smith, M.H., Ploegh, H.L., Weissman, J.S. Road to ruin: Targeting proteins for degradation in the endoplasmic reticulum. Science, 334: 1086-90, 2011. (PMID: 22116878 / PMCID in process)

87. Sugihara, J., Smirnova, I., Kasho, V., Kaback, HR. 2011. Sugar recognition by CscB and LacY. Biochemistry 50(51): 11009-11014. (PMID: 22106930/PMCID: PMC3249425)

88. Aquila, A., Hunter, M. S., Doak, R. B., Kirian, R. A., Fromme, P., White, T. A., Andreasson, J., Arnlund, D., Bajt, S. a., Barends, T. R. M., Barthelmess, M., Bogan, M. J., Bostedt, C., Bottin, H., Bozek, J. D., Caleman, C., Coppola, N., Davidsson, J., DePonte, D. P., Elser, V., Epp, S. W., Erk, B., Fleckenstein, H., Foucar, L., Frank, M., Fromme, R., Graafsma, H., Grotjohann, I., Gumprecht, L., Hajdu, J., Hampton, C. Y., Hartmann, A., Hartmann, R., Hau-Riege, S., Hauser, G., Hirsemann, H., Holl, P., Holton, J. M., Hömke, A., Johansson, L., Kimmel, N., Kassemeyer, S., Krasniqi, F., Kühnel, K.-U., Liang, M., Lomb, L., Malmerberg, E., Marchesini, S., Martin, A. V., Maia, F. R. N. C., Messerschmidt, M., Nass, K., Reich, C., Neutze, R., Rolles, D., Rudek, B., Rudenko, A., Schlichting, I., Schmidt, C., Schmidt, K. E., Schulz, J., Seibert, M. M., Shoeman, R. L., Sierra, R., Soltau, H., Starodub, D., Stellato, F., Stern, S., Strüder, L., Timneanu, N., Ullrich, J., Wang, X., Williams, G. J., Weidenspointner, G., Weierstall, U., Wunderer, C., Barty, A., Spence, J. C. H. & Chapman, H. N. Time-resolved protein nanocrystallography using an X-ray free-electron laser. Opt. Express 20, 2706-2716, 2012. (PMID: 22330507 / PMCID in process)

89. Frost, A., Elgort, M.G., Brandman, O., Ives, C., Collins, S.R., Miller-Vedam, L., Weibezahn, J., Hein, M.Y., Poser, I., Mann, M., Hyman, A.A., Weissman, J.S. (2012).  Comparing S. pombe and S. cerevisiae Genetic Interactions Reveals  Functional Repurposing and Identifies New Organelle Homeostasis and  Mitosis Control Genes. Cell, in press (June 8, 2012 issue)

90. Jiang, X., Guan, L., Zhou, Y., Hong, W-X., Zhang, Q., Kaback, H.R.  Evidence for an intermediate conformer of LacY.  Proc Natl Acad Sci USA, doi/10.1073, 2012. (PMID: 22355148/PMCID: PMC3311394)

91. Zhou, Y., Madej, G., Guan, L., Nie, Y., Kaback, H.R. An Early Event in the Transport Mechanism of LacY Protein Interaction between helices v and i. J of Biological Chemistry 286(35): 30415-30422, 2012. (PMID: 21730060/PMCID: PMC3162401)

92. Chaudhary, S., Pak, J.E., Gruswitz, F., Sharma, V., Stroud, R.M. (2012). Overexpressing Human Membrane Proteins in Stably Transfected and Clonal Human Embryonic Kidney 293S Cells. Nature Protocols 7 453-466. (PMID: 22322218)

93. Varrin-Doyer, M., Spencer, C.M., Schulze-Topphoff, U., Nelson, P.A., Stroud, R.M., Cree, B.A., Zamvil, S.S. (2012). Aquaporin 4-specific T cells in neuromyelitis optica exhibit a Th17 bias and recognize Clostridium ABC transporter. Ann Neurol 72: 53-64. (PMCID: PMC3405197)

94. Wu, S., Avila-Sakar, A., Kim, J., Booth, D.S., Greenberg, C.H., Rossi, A., Liao, M., Li, X., Alian, A., Griner, S.L., Juge, N., Yu, Y., Mergel, C.M., Chaparro-Riggers, J., Strop, P., Tampe, R., Edwards, R.H., Stroud, R.M., Craik, C.S., Cheng, Y. (2012) Fabs enable single particle cryoEM studies of small proteins. Structure 20: 582-92. PMCID: PMC3322386

95. Pedersen, B.P., Kumar, H., Waight, A.B., Risenmay, A., Roe-Zurz, Z., Chau, B., Schlessinger, A., Harries, W., Sali, A., Johri, A.K., Stroud, R.M. (2013). Crystal structure of a eukaryotic phosphate transporter. Nature. (PMID: 23542591)

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